Chemotaxis of Arbacia punctulata spermatozoa to resact, a peptide from the egg jelly layer

نویسندگان

  • G E Ward
  • C J Brokaw
  • D L Garbers
  • V D Vacquier
چکیده

Resact, a peptide of known sequence isolated from the jelly layer of Arbacia punctulata eggs, is a potent chemoattractant for A. punctulata spermatozoa. The chemotactic response is concentration dependent, is abolished by pretreatment of the spermatozoa with resact, and shows an absolute requirement for millimolar external calcium. A. punctulata spermatozoa do not respond to speract, a peptide isolated from the jelly layer of Strongylocentrotus purpuratus eggs. This is the first report of animal sperm chemotaxis in response to a defined egg-derived molecule.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Assessment of sperm chemokinesis with exposure to jelly coats of sea urchin eggs and resact: a microfluidic experiment and numerical study.

Specific peptides contained within the extracellular layer, or jelly coat, of a sea urchin egg have been hypothesized to play an important role in fertilization, though separate accounting of the effects of chemoattraction, chemokinesis, sperm agglomeration and the other possible roles of the jelly coat have not been reported. In the present study, we used a microfluidic device that allowed det...

متن کامل

Towards Understanding the Molecular Mechanism of Sperm Chemotaxis

The awareness that, in most animal species, the prospects of the male's spermatozoa to reach the female's eggs are very slim in the absence of some guidance mechanism (usually chemical in nature) has been acquired gradually; first, in marine species, where both types of gametes are released into sea water (for review see Miller, 1985), and ultimately, in the last decade or so, in mammals (for r...

متن کامل

Revisiting the Role of H+ in Chemotactic Signaling of Sperm

Chemotaxis of sperm is an important step toward fertilization. During chemotaxis, sperm change their swimming behavior in a gradient of the chemoattractant that is released by the eggs, and finally sperm accumulate near the eggs. A well established model to study chemotaxis is the sea urchin Arbacia punctulata. Resact, the chemoattractant of Arbacia, is a peptide that binds to a receptor guanyl...

متن کامل

Fluid-Structure Interaction Analysis of Flow-Induced Deformation in a Two-Phase, Neo-Hookean Marine Egg

Coupled computational fluid dynamics and finite element analyses were used to determine the material properties of the egg and jelly layer of the sea urchin Arbacia punctulata. Prior experimental shear flow results were used to provide material parameters for these simulations. A Neo-Hookean model was used to model the hyperelastic behaviors of the jelly layer and egg. A simple compressive simu...

متن کامل

Different Migration Patterns of Sea Urchin and Mouse Sperm Revealed by a Microfluidic Chemotaxis Device

Chemotaxis refers to a process whereby cells move up or down a chemical gradient. Sperm chemotaxis is known to be a strategy exploited by marine invertebrates such as sea urchins to reach eggs efficiently in moving water. Less is understood about how or whether chemotaxis is used by mammalian sperm to reach eggs, where fertilization takes place within the confinement of a reproductive tract. In...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • The Journal of Cell Biology

دوره 101  شماره 

صفحات  -

تاریخ انتشار 1985